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Trinity College Dublin

Sequencing of White clover

Research Field: 
Life Sciences
Resource Type: 
Dedicated human Support
Resource Class: 
C
Lead PI: 
Susanne Barth
Abstract: 
White clover (Trifolium repens L.) is a perennial legume that is important in grasslands as forage and feed for sheep and cattle, and as a natural source of soil nitrogen. White clover is self-incompatible, meaning that a plant producing male and female gametes cannot self-fertilise due to a biochemical incompatibility. The aim of this project is to find out more about the mechanism controlling self-incompatibility in white clover. During this project, we have used genetic linkage mapping to locate the white clover self-incompatibility locus on the distal end of homologue 1, which correlates to a similar result in red clover and demonstrates that only a single locus is involved in this species. Using degenerate primers and next-generation sequencing (454) of the transcribed genes in the female reproductive organs, we are trying to identify candidates for one of the genes involved in self-incompatibility in white clover. Knowledge of the mechanism of self-incompatibility in white clover would contribute to the study of self-incompatibility in general, which is highly prevalent in flowering plants. It could also be of help to clover breeders. I contacted the TCHPC group in relation to 454 sequencing data from thewhite clover pistil (female reproductive tissue) transcriptome, which has not been sequenced before. This kind of data in general is relatively new and I wanted advice on analysis tools. The group were very helpful.
Start Date: 
09/2010
Duration: 
3.00months

Last updated 08 Sep 2010Contact TCHPC: info | support.